Daliya Pencheva
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| Poster 0317 | Friday 13.06.25 09:30 AM Clinical Poster Tours: Systemic lupus erythematosus II Author: Andrea Fava (USA) Title: The immune map of lupus nephritis: a spatially-resolved kidney proteomic approach  This study developed an innovative 18-plex serial immunohistochemistry workflow to map the spatial organization of immune cells in lupus nephritis kidney biopsies. Using advanced AI-assisted analysis on 29 kidney biopsies containing nearly 2 million cells, researchers identified over 12,000 cellular aggregates and discovered distinct immune cell structures in different kidney regions.The findings revealed that while most aggregates were small, medium and large aggregates contained one-third of all immune cells and showed different compositions related to disease severity. Glomerular aggregates were primarily composed of myeloid cells and associated with protein levels, while tubulointerstitial aggregates showed greater diversity and correlated with kidney function. This spatial mapping approach provides new insights into lupus nephritis pathogenesis and potential therapeutic targets. |